Tributyrate as a substrate for the determination of lipase activity in milk

Abstract

SummaryA procedure is described for the determination of the lipolytic activity in milk using tributyrate as substrate. The method is based on the titration of liberated butyric acid in a diethyl ether: light petroleum (2·75:1, v/v) extract of the incubation mixture. Some of the fundamental factors involved in the procedure are discussed.