Comparison and Development of CLIA and ELISA for Detection of Sudan I in Food Sample

Abstract

Two immunological methods, ELISA and CLIA were optimized and compared. The sensitivity of CLIA was higher. The optimized CL-ELISA allowed the Sudan I detection in a linear range of 0.156-5 ng mL-1, the IC50 was 0.642ng mL-1 and the limit of detection (LOD) was 0.05ng mL-1. The method showed good recoveries with spiked chili powder. The recovery rate range from 97.28-112.39%. The proposed method proved to be efficient for the detection of Sudan I in food samples.