Affiliation:
1. Department of Biochemistry, University of Kentucky, Lexington, Kentucky 40506
Abstract
Seven inositol-containing phospholipids in
Saccharomyces cerevisiae
can be resolved by two-dimensional chromatography on silicic acid-impregnated paper. Four of these lipids are stable to mild alkaline methanolysis; one of these has already been characterized as a mannosyl di(inositolphosphoryl) ceramide. Addition of labeled 2-deoxy-
d
-glucose to a growing culture results in label appearing in five lipids, some of which are alkali-stable and have the same
R
F
values as the alkali-stable inositol-containing phospholipids. These lipids are labeled rapidly. The deoxyglucose is incorporated intact, probably in a glycosidic link, since the deoxyglucose is released by mild acid treatment.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
138 articles.
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