Affiliation:
1. Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Czechoslovakia
Abstract
The growth inhibition and the lysis of
Saccharomyces cerevisiae
caused by 2-deoxy-
d
-glucose (2-DG) were shown to be a consequence of unbalanced cellular growth and division. The lysis, but not the repression of growth and osmotic fragility of cells, could be suppressed by the addition of mannitol as an osmotic stabilizer. This result, as well as the morphological changes observed in the cells and changes in the chemical composition of the cell walls, showed that
S. cerevisiae
grown in the presence of 2-DG formed weakened cell walls responsible for the osmotic fragility. Evidence is presented for the first time demonstrating the incorporation of 2-DG into yeast cell wall material. Other data suggest that the inhibition of yeast growth by 2-DG results from an interference of phosphorylated metabolites of 2-DG with metabolic processes of glucose and mannose involved in the synthesis of structural cell wall polysaccharides.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
74 articles.
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