Inactivation of Cryptosporidium parvum Oocysts in Fresh Apple Cider by UV Irradiation-Reference-Cited by-同舟云学术

Inactivation of Cryptosporidium parvum Oocysts in Fresh Apple Cider by UV Irradiation

Published:2002-08 Issue:8 Volume:68 Page:4168-4172
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Hanes D. E.¹,Worobo R. W.²,Orlandi P. A.³,Burr D. H.¹,Miliotis M. D.³,Robl M. G.¹,Bier J. W.³,Arrowood M. J.⁴,Churey J. J.²,Jackson G. J.³

Affiliation:

1. Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, Laurel, Maryland

2. Cornell University Department of Food Science and Technology, New York State Agricultural Experiment Station, Geneva, New York

3. U.S. Food and Drug Administration, Washington, D.C.

4. Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

Abstract

ABSTRACT This study evaluated the efficacy of UV irradiation on the inactivation of Cryptosporidium parvum oocysts in fresh apple cider. Cider was inoculated with oocysts and exposed to 14.32 mJ of UV irradiation/cm 2 . Oocyst viability was assessed with the gamma interferon gene knockout (GKO) mouse and infant BALB/cByJ mouse models. All GKO mice challenged with UV-treated cider demonstrated no morbidity or mortality, and infant BALB/c mice challenged with treated cider were negative for the presence of C. parvum . In contrast, the GKO mice challenged with non-UV-treated inoculated cider died and the parasite was detected in the ileums of all challenged infant mice. This study shows that UV irradiation can be used to inactivate C. parvum in fresh apple cider.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.68.8.4168-4172.2002

Reference27 articles.

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