d -Methionine and d -Phenylalanine Improve Lactococcus lactis F44 Acid Resistance and Nisin Yield by Governing Cell Wall Remodeling-Reference-Cited by-同舟云学术

d -Methionine and d -Phenylalanine Improve Lactococcus lactis F44 Acid Resistance and Nisin Yield by Governing Cell Wall Remodeling

Published:2020-04-17 Issue:9 Volume:86 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Wu Hao¹²,Xue Ershu¹²,Zhi Ning¹²,Song Qianqian¹²,Tian Kairen¹²,Caiyin Qinggele¹²,Yuan Lin¹,Qiao Jianjun¹²³

Affiliation:

1. Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China

2. Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin, China

3. SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, China

Abstract

As d -amino acids play an important role in cell wall synthesis, we analyzed the effects of 19 different d -amino acids on L. lactis F44, demonstrating that d -Met and d -Phe can participate in peptidoglycan (PG) synthesis and improve the acid resistance and nisin yield of this strain. murF overexpression further increased the levels of d -Met and d -Phe incorporated into PG and contributed to the acid resistance of the strain. These findings suggest that d -Met and d -Phe can be incorporated into PG to improve the acid resistance and nisin yield of L. lactis , and this study provides new ideas for the enhancement of nisin production.

Funder

the funds for creative research groups of China

the national key R&D program of China

the national natural science foundation of China

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02981-19

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1. 10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis