New Granada Medium for detection and identification of group B streptococci

Author:

de la Rosa M1,Perez M1,Carazo C1,Pareja L1,Peis J I1,Hernandez F1

Affiliation:

1. Microbiology Service, Virgen de las Nieves Hospital, Granada, Spain.

Abstract

A methotrexate-containing medium for the detection of beta-hemolytic group B streptococci from clinical specimens on the basis of detection of pigment is described. The medium contained peptone, starch, serum, MgSO4, glucose, pyruvate, methotrexate (as pigment enhancer), phosphate-morpholine-propanesulfonic acid buffer, and selective agents. The recovery of beta-hemolytic group B streptococci was comparable to that obtained with selective broth.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference16 articles.

1. Comparison of commercially available group B streptococcal latex agglutination assays;Ascher D. P.;J. Clin. Microbiol.,1991

2. Granada medium for detection and identification of group B streptococci;de la Rosa M.;J. Clin. Microbiol.,1983

3. Edwards M. S. and C. J. Baker. 1990. Streptococcus agalactiae (group B streptococcus) p. 1554-1563. In G. L. Mandel R. G. Douglas and J. E. Bennett (ed.) Principles and practice of infectious diseases 3rd ed. Churchill Livingstone New York.

4. Facklam R. R. and J. A. Washington II. 1991. Streptococcus and related catalase-negative gram-positive cocci p. 238-257. In A. Balows W. J. Hausler Jr. K. L. Herrmann H. D. Isenberg and H. J. Shadomy (ed.) Manual of clinical microbiology 5th ed. American Society for Microbiology Washington D.C.

5. Islam A. K. M. 1977. Rapid recognition of group B streptococci. Lancet i:256-257.

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