Comparison of Rectoanal Mucosal Swab Cultures and Fecal Cultures for Determining Prevalence of Escherichia coli O157:H7 in Feedlot Cattle

Author:

Greenquist M. A.1,Drouillard J. S.1,Sargeant J. M.2,Depenbusch B. E.1,Shi Xiaorong2,Lechtenberg K. F.3,Nagaraja T. G.2

Affiliation:

1. Department of Animal Sciences and Industry

2. Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, Kansas 66506

3. Midwest Veterinary Services, Inc., Oakland, Nebraska 68045

Abstract

ABSTRACT We compared fecal samples with samples collected with rectoanal mucosa swabs (RAMS) to determine the prevalence of Escherichia coli O157 in feedlot cattle ( n = 747). Escherichia coli O157 was detected in 9.5% of samples collected with RAMS and 4.7% of samples tested by fecal culture. Pulsed-field gel electrophoresis analysis of isolates suggested that the strains colonizing the rectoanal junction were the same as those from the feces. Mucosal swab sampling was more sensitive than fecal sampling for determining the prevalence of E. coli O157 in feedlot cattle.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference24 articles.

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