F 420 H 2 Is Required for Phthiocerol Dimycocerosate Synthesis in Mycobacteria

Abstract

ABSTRACT Phthiocerol dimycocerosates (PDIM) are a group of cell surface-associated apolar lipids of Mycobacterium tuberculosis and closely related mycobacteria, such as Mycobacterium bovis and Mycobacterium leprae . A characteristic methoxy group of these lipids is generated from the methylation of a hydroxyl group of the direct precursors, the phthiotriols. The precursors arise from the reduction of phthiodiolones, the keto intermediates, by a ketoreductase. The putative phthiodiolone ketoreductase (PKR) is encoded by Rv2951c in M. tuberculosis and BCG_2972c in M. bovis BCG, and these open reading frames (ORFs) encode identical amino acid sequences. We investigated the cofactor requirement of the BCG_2972c protein. A comparative analysis based on the crystallographic structures of similar enzymes identified structural elements for binding of coenzyme F 420 and hydrophobic phthiodiolones in PKR. Coenzyme F 420 is a deazaflavin coenzyme that serves several key functions in pathogenic and nonpathogenic mycobacteria. We found that an M. bovis BCG mutant lacking F 420 -dependent glucose-6-phosphate dehydrogenase (Fgd), which generates F 420 H 2 (glucose-6-phosphate + F 420 → 6-phosphogluconate + F 420 H 2 ), was devoid of phthiocerols and accumulated phthiodiolones. When the mutant was provided with F 420 H 2 , a broken-cell slurry of the mutant converted accumulated phthiodiolones to phthiocerols; F 420 H 2 was generated in situ from F 420 and glucose-6-phosphate by the action of Fgd. Thus, the reaction mixture was competent in reducing phthiodiolones to phthiotriols (phthiodiolones + F 420 H 2 → phthiotriols + F 420 ), which were then methylated to phthiocerols. These results established the mycobacterial p hthiodiolone k eto r eductase as an F 420 H 2 -dependent enzyme (fPKR). A phylogenetic analysis of close homologs of fPKR revealed potential F 420 -dependent lipid-modifying enzymes in a broad range of mycobacteria. IMPORTANCE Mycobacterium tuberculosis is the causative agent of tuberculosis, and phthiocerol dimycocerosates (PDIM) protect this pathogen from the early innate immune response of an infected host. Thus, the PDIM synthesis system is a potential target for the development of effective treatments for tuberculosis. The current study shows that a PDIM synthesis enzyme is dependent on the coenzyme F 420 . F 420 is universally present in mycobacteria and absent in humans. This finding expands the number of experimentally validated F 420 -dependent enzymes in M. tuberculosis to six, each of which helps the pathogen to evade killing by the host immune system, and one of which activates an antituberculosis drug, PA-824. This work also has relevance to leprosy, since similar waxy lipids are found in Mycobacterium leprae .