Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Can Accurately Differentiate between Mycobacterium masilliense (M. abscessus subspecies bolletti) and M. abscessus ( Sensu Stricto )-Reference-Cited by-同舟云学术

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Can Accurately Differentiate between Mycobacterium masilliense (M. abscessus subspecies bolletti) and M. abscessus ( Sensu Stricto )

Published:2013-09 Issue:9 Volume:51 Page:3113-3116
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Teng Shih-Hua¹²,Chen Chung-Ming³⁴,Lee Meng-Rui⁵⁶,Lee Tai-Fen³,Chien Kun-Yi¹,Teng Lee-Jene³⁷,Hsueh Po-Ren³⁵

Affiliation:

1. Department of Graduate Institute of Biomedical Sciences, Chang Gung University, Tao-Yuan, Taiwan

2. Bruker Taiwan Co., Ltd., Taipei, Taiwan

3. Departments of Laboratory Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan

4. Pediatrics, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan

5. Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan

6. Department of Internal Medicine, Taoyuan General Hospital, Taoyuan County, Taiwan

7. Department and Graduate Institute of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University, Taipei, Taiwan

Abstract

ABSTRACT Among 36 Mycobacterium masilliense and 22 M. abscessus isolates identified by erm (41) PCR and sequencing analysis of rpoB and 23S rRNA genes, the rate of accurate differentiation between these two subspecies was 100% by cluster analysis of spectra generated by Bruker Biotyper matrix-assisted laser desorption ionization–time of flight mass spectrometry.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01239-13

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