Identification of Mycobacterium abscessus using the peaks of ribosomal protein L29, L30 and hemophore-related protein by MALDI-MS proteotyping-Reference-Cited by-同舟云学术

Identification of Mycobacterium abscessus using the peaks of ribosomal protein L29, L30 and hemophore-related protein by MALDI-MS proteotyping

Published:2024-05-16 Issue:1 Volume:14 Page:
ISSN:2045-2322
Container-title:Scientific Reports
language:en
Short-container-title:Sci Rep

Author:

Takei Satomi,Teramoto Kanae,Sekiguchi Yuji,Ihara Hiroaki,Tohya Mari,Iwamoto Shinichi,Tanaka Koichi,Khasawneh Abdullah,Horiuchi Yuki,Misawa Shigeki,Naito Toshio,Kirikae Teruo,Tada Tatsuya,Tabe Yoko

Abstract

AbstractMycobacteroides (Mycobacterium) abscessus, which causes a variety of infectious diseases in humans, is becoming detected more frequently in clinical specimens as cases are spreading worldwide. Taxonomically, M. abscessus is composed of three subspecies of M. abscessus subsp. abscessus, M. abscessus subsp. bolletii, and M. abscessus subsp. massiliense, with different susceptibilities to macrolides. In order to identify rapidly these three subspecies, we determined useful biomarker proteins, including ribosomal protein L29, L30, and hemophore-related protein, for distinguishing the subspecies of M. abscessus using the matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) profiles. Thirty-three clinical strains of M. abscessus were correctly identified at the subspecies-level by the three biomarker protein peaks. This study ultimately demonstrates the potential of routine MALDI-MS-based laboratory methods for early identification and treatment for M. abscessus infections.

Funder

Japan Society for the Promotion of Science

Publisher

Springer Science and Business Media LLC

Link

https://www.nature.com/articles/s41598-024-61549-7.pdf

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