Significantly enhancing recombinant alkaline amylase production in Bacillus subtilis by integration of a novel mutagenesis-screening strategy with systems-level fermentation optimization-Reference-Cited by-同舟云学术

Significantly enhancing recombinant alkaline amylase production in Bacillus subtilis by integration of a novel mutagenesis-screening strategy with systems-level fermentation optimization

Published:2016-10-17 Issue:1 Volume:10 Page:
ISSN:1754-1611
Container-title:Journal of Biological Engineering
language:en
Short-container-title:J Biol Eng

Author:

Ma Yingfang,Shen Wei,Chen Xianzhong,Liu Long,Zhou Zhemin,Xu Fei,Yang Haiquan

Funder

863 Program

Natural Science Foundation of Jiangsu Province

National Natural Science Foundation of China (CN)

the Open Project Program of the Key Laboratory of Industrial Biotechnology, Ministry of Education, China

111 Project

Publisher

Springer Science and Business Media LLC

Subject

Cell Biology,Molecular Biology,Biomedical Engineering,Environmental Engineering

Link

http://link.springer.com/content/pdf/10.1186/s13036-016-0035-2.pdf

Reference33 articles.

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3. Vester JK, Glaring MA, Stougaard P. An exceptionally cold-adapted alpha-amylase from a metagenomic library of a cold and alkaline environment. Appl Microbiol Biotechnol. 2015;99:717–27.

4. Murakami S, Nagasaki K, Nishimoto H, Shigematu R, Umesaki J, Takenaka S, Kaulpiboon J, Prousoontorn M, Limpaseni T, Pongsawasdi P, Aoki K. Purification and characterization of five alkaline, thermotolerant, and maltotetraose-producing alpha-amylases from Bacillus halodurans MS-2-5, and production of recombinant enzymes in Escherichia coli. Enzym Microb Technol. 2008;43:321–8.

5. Yang HQ, Liu L, Shin H-D, Chen RR, Li JH, Du GC, Chen J. Integrating terminal truncation and oligopeptide fusion for a novel protein engineering strategy to improve specific activity and catalytic efficiency: alkaline alpha-amylase as a case study. Appl Environ Microbiol. 2013;79:6429–38.

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