Amplified detection of nucleic acids and proteins using an isothermal proximity CRISPR Cas12a assay-Reference-Cited by-同舟云学术

Amplified detection of nucleic acids and proteins using an isothermal proximity CRISPR Cas12a assay

Published:2021 Issue:6 Volume:12 Page:2133-2137
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Li Yongya¹²³⁴⁵,Mansour Hayam⁶⁷⁸⁹¹⁰,Watson Colton J. F.¹¹⁸⁹¹⁰,Tang Yanan¹²³⁴⁵,MacNeil Adam J.¹¹⁸⁹¹⁰,Li Feng¹²³⁴⁵^ORCID

Affiliation:

1. Key Laboratory of Green Chemistry & Technology of Ministry of Education

2. College of Chemistry

3. Analytical & Testing Center

4. Sichuan University

5. Chengdu

6. Department of Chemistry

7. Centre for Biotechnology

8. Brock University

9. St. Catharines

10. Canada

11. Department of Health Sciences

Abstract

Herein, we develop an isothermal proximity CRISPR Cas12a assay that harnesses the target-induced collateral cleavage activity of Cas12a for the quantitative profiling of gene expression and detection of proteins with high sensitivity and specificity.

Funder

Fundamental Research Funds for the Central Universities

Natural Sciences and Engineering Research Council of Canada

Ontario Ministry of Research, Innovation and Science

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/SC/D0SC06113A

Reference27 articles.