Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6-Reference-Cited by-全球学者库

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

Published:2018-04-27 Issue:6387 Volume:360 Page:439-444
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Gootenberg Jonathan S.¹²³⁴⁵^ORCID,Abudayyeh Omar O.¹²³⁴⁶^ORCID,Kellner Max J.¹^ORCID,Joung Julia¹²³⁴,Collins James J.¹⁴⁶⁷⁸,Zhang Feng¹²³⁴^ORCID

Affiliation:

1. Broad Institute of the Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.

2. McGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA.

3. Department of Brain and Cognitive Science, MIT, Cambridge, MA 02139, USA.

4. Department of Biological Engineering, MIT, Cambridge, MA 02139, USA.

5. Department of Systems Biology, Harvard University, Boston, MA 02115, USA.

6. Department of Health Sciences and Technology, MIT, Cambridge, MA 02139, USA.

7. Institute for Medical Engineering and Science, MIT, Cambridge, MA 02139, USA.

8. Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.

Abstract

Taking CRISPR technology further CRISPR techniques are allowing the development of technologies for nucleic acid detection (see the Perspective by Chertow). Taking advantages of the distinctive enzymatic properties of CRISPR enzymes, Gootenberg et al. developed an improved nucleic acid detection technology for multiplexed quantitative and highly sensitive detection, combined with lateral flow for visual readout. Myhrvold et al. added a sample preparation protocol to create a field-deployable viral diagnostic platform for rapid detection of specific strains of pathogens in clinical samples. Cas12a (also known as Cpf1), a type V CRISPR protein, cleaves double-stranded DNA and has been adapted for genome editing. Chen et al. discovered that Cas12a also processes single-stranded DNA threading activity. A technology platform based on this activity detected human papillomavirus in patient samples with high sensitivity. Science , this issue p. 439 , p. 444 , p. 436 ; see also p. 381

Funder

National Institutes of Health

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference27 articles.

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2. C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

3. Nucleic acid detection with CRISPR-Cas13a/C2c2

4. Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection

5. RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes

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