Nucleic acid detection with CRISPR-Cas13a/C2c2-Reference-Cited by-同舟云学术

Nucleic acid detection with CRISPR-Cas13a/C2c2

Published:2017-04-28 Issue:6336 Volume:356 Page:438-442
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Gootenberg Jonathan S.¹²³⁴⁵^ORCID,Abudayyeh Omar O.¹²³⁴⁶^ORCID,Lee Jeong Wook⁷^ORCID,Essletzbichler Patrick¹²³⁴^ORCID,Dy Aaron J.¹⁴⁸^ORCID,Joung Julia¹²³⁴,Verdine Vanessa¹²³⁴^ORCID,Donghia Nina⁷,Daringer Nichole M.⁸^ORCID,Freije Catherine A.¹⁹^ORCID,Myhrvold Cameron¹⁹^ORCID,Bhattacharyya Roby P.¹^ORCID,Livny Jonathan¹^ORCID,Regev Aviv¹¹⁰^ORCID,Koonin Eugene V.¹¹^ORCID,Hung Deborah T.¹^ORCID,Sabeti Pardis C.¹⁹¹²¹³^ORCID,Collins James J.¹⁴⁶⁷⁸^ORCID,Zhang Feng¹²³⁴^ORCID

Affiliation:

1. Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.

2. McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA.

3. Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

4. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

5. Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.

6. Department of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

7. Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.

8. Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

9. Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA.

10. Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

11. National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA.

12. Department of Immunology and Infectious Disease, Harvard School of Public Health, Boston, MA 02115, USA.

13. Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA.

Abstract

Sensitive and specific CRISPR diagnostics Methods are needed that can easily detect nucleic acids that signal the presence of pathogens, even at very low levels. Gootenberg et al. combined the allele-specific sensing ability of CRISPR-Cas13a with recombinase polymerase amplification methods to detect specific RNA and DNA sequences. The method successfully detected attomolar levels of Zika virus, as well as the presence of pathogenic bacteria. It could also be used to perform human genotyping from cell-free DNA. Science , this issue p. 438

Funder

Howard Hughes Medical Institute

NIH Office of the Director

NSF Office of the Director

Simons Foundation

Paul G. Allen Family Foundation

New York Stem Cell Foundation

Vallee Foundation

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary