Genotyping simplified: rationally designed antisense oligonucleotide-mediated PCR amplification-free colorimetric sensing of viral RNA in HCV genotypes 1 and 3-Reference-Cited by-同舟云学术

Genotyping simplified: rationally designed antisense oligonucleotide-mediated PCR amplification-free colorimetric sensing of viral RNA in HCV genotypes 1 and 3

Published:2021 Issue:15 Volume:146 Page:4767-4774
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Mohammed Almas Shamaila¹²³⁴⁵,Balapure Aniket⁶²³⁴^ORCID,Khan Aleem Ahmed⁷⁸⁹⁴,Khaja Mahammad Nanne⁵¹⁰⁴,Ganesan Ramakrishnan⁶²³⁴^ORCID,Dutta Jayati Ray¹²³⁴^ORCID

Affiliation:

1. Department of Biological Sciences

2. BITS Pilani Hyderabad Campus

3. Hyderabad-500078

4. India

5. Bioviz Technologies Pvt. Ltd

6. Department of Chemistry

7. Centre for Liver Research & Diagnostics

8. Deccan College of Medical Sciences

9. Hyderabad-500058

10. Hyderabad-500034

Abstract

A proof-of-concept PCR-free colorimetric and spectrophotometric hepatitis C viral genotyping of 1 and 3 in clinical cohorts using a combination of plasmonic Au NPs and non-thiolated genotype-specific antisense oligonucleotides is presented.

Funder

Birla Institute of Technology and Science, Pilani

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/AN/D1AN00590A

Reference36 articles.

1. Comparison of Abbott RealTime HCV Genotype II with Versant Line Probe Assay 2.0 for Hepatitis C Virus Genotyping

2. Simplified Hepatitis C Virus Genotyping by Heteroduplex Mobility Analysis

3. Comparison of plasma virus loads among individuals infected with hepatitis C virus (HCV) genotypes 1, 2, and 3 by quantiplex HCV RNA assay versions 1 and 2, Roche Monitor assay, and an in-house limiting dilution method