Precise, wide field, and low-cost imaging and analysis of core–shell beads for digital polymerase chain reaction

Author:

Yadav Ajeet Singh1,Galogahi Fariba Malekpour1,Tran Du Tuan1,Vashi Aditya1,Ooi Chin Hong1ORCID,Rajan Sreejith Kamalalayam1,Nguyen Nam-Trung1ORCID

Affiliation:

1. Queensland Micro- and Nanotechnology Centre, Griffith University, 170 Kessels Road, Nathan, QLD 4111, Australia

Abstract

This study introduces a core–shell bead-based digital PCR platform, where PCR mix is enclosed in microfluidic beads. Multiple flow focusing stages in the device facilitate bead formation. Results of digital PCR are effectively compared with RT-qPCR.

Funder

Australian Research Council

Griffith University

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

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