The applicability of digital PCR for the assessment of detection limits in GMO analysis

Author:

Burns M. J.,Burrell A. M.,Foy C. A.

Publisher

Springer Science and Business Media LLC

Subject

Industrial and Manufacturing Engineering,Biochemistry,General Chemistry,Food Science,Biotechnology

Reference14 articles.

1. Burns M (Ed) EU Co-Extra Task 4.2 report (2009) Procedure for the experimental design and validation of novel methods and guidelines for data processing, method validation and good data handling practices. CO-EXTRA: GM and non-GM supply chains: their CO-EXistence and TRAceability. Project number: 007158, Integrated project, Sixth Framework Programme, Priority 5, Food Quality and Safety. http://www.coextra.eu/deliverables/deliverable1333.pdf

2. Burns M, Corbisier P, Wiseman G, Valdivia H, McDonald P, Bowler P, Ohara K, Schimmel H, Charels D, Damant A, Harris N (2006) Comparison of plasmid and genomic DNA calibrants for the quantification of genetically modified ingredients. Eur Food Res Technol 224(2):249–258

3. Burns M, Valdivia H, Harris N (2004) Analysis and interpretation of data from real-time PCR trace detection methods using quantitation of GM soya as a model system. Anal Bioanal Chem 378(6):1616–1623

4. BioMark Digital PCR Analysis Software User Guide. Fluidigm (Fluidigm Corporation, San Francisco)

5. Bhat S, Herrmann J, Armishaw P, Corbisier P, Kerry ER (2009) Single molecule detection in nanofluidic digital array enables accurate measurement of DNA copy number. Anal Bioanal Chem. doi: 10.1007/s00216-009-2729-5

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