Rapid, specific and sensitive detection of <i>Vibrio parahaemolyticus</i> in seafood by accelerated strand exchange amplification-Reference-Cited by-同舟云学术

Rapid, specific and sensitive detection of Vibrio parahaemolyticus in seafood by accelerated strand exchange amplification

Published:2023 Issue:5 Volume:15 Page:655-662
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Sun Ritong¹,Chen Jiao¹,Wang Yingeng²,Zhang Zheng²,Li Yong¹,Li Fengmei¹,Ma Cuiping¹^ORCID,Han Qingxia³,Shi Yanjing¹^ORCID

Affiliation:

1. Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Nucleic Acid Rapid Detection Engineering Research Center, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China

2. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, PR China

3. Bin Zhou Polytechnic, Qingdao, 256600, PR China

Abstract

The total detection time from DNA preparation was about 20 min. The LOD of the ASEA method was 25 CFU mL−1 of pure cultured V. parahaemolyticus. The enrichment time was significantly reduced to 3 h with a LOD of 18 CFU g−1 of spiked scallop meat.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Shandong Province

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/AY/D2AY01889F

Reference35 articles.

1. Vibrio vulnificus: new insights into a deadly opportunistic pathogen

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3. Delineating the Origins of Vibrio parahaemolyticus Isolated from Outbreaks of Acute Hepatopancreatic Necrosis Disease in Asia by the Use of Whole Genome Sequencing

4. Survival of foodborne pathogens ( Escherichia coli O157:H7, Salmonella Typhimurium, Staphylococcus aureus , Listeria monocytogenes , and Vibrio parahaemolyticus ) in raw ready-to-eat crab marinated in soy sauce

5. Seafood pathogens and information on antimicrobial resistance: A review

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