Analysis of the Fungi Community Variation during Rice Storage through High Throughput Sequencing

Abstract

Rice storage conditions include location, granary depth, storage time, temperature and atmosphere. The fungi community varies during storage, but how these communities change remains unexplored so far. This study collected rice samples from granaries in different horizontal and vertical directions and storage time over two years. High-throughput ITS (Internal Transcribed Spacer) sequencing analysis revealed that Ascomycota (73.81%), Basidiomycota (6.56%) and Mucoromycota (9.42%) were the main Eumycota present during rice storage. The main fungi communities were Aspergillus sp., Fusarium sp., Rhizopus sp., Gibberella sp., Tilletia sp. and Penicillium sp. The contribution of storage time, horizontal orientation and vertical depth effect on fungi community relative abundance were 17.18%, 5.98% and 0.11%, respectively. Aspergillus sp. was the predominant Eubacterium during this process. The horizontal A was mainly occupied by Paraconiothyrium sp. and the location S, had Clavispora sp. Both of these varied dramatically during storage. Furthermore, Aspergillus sp., as a main mycotoxin producer, was the dominant fungi at vertical L1. This study comprehensively analyzed fungi community variation in horizontal and vertical directions to elucidate fungi community variation on rice during storage and to find the detrimental fungi. Therefore, it is important to improve granary ventilation systems and to ensure a uniform atmosphere to control fungi growth.