Affiliation:
1. School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi’an 710021, China
2. School of Chemistry and Chemical Engineering, Xi’an University of Science and Technology, Xi’an 710054, China
Abstract
From Siraitia grosvenorii, a natural polysaccharide named SGP-1 was discovered, and its purity was determined to be 96.83%. Its structure is a glucan with 4-, 6- and 4,6-linked glucose units. In this paper, the sulfated derivative S-SGP of SGP-1 was prepared by the chlorosulfonic acid method. The sulfated derivatives were analyzed by Fourier transform infrared spectroscopy (FT-IR), gel permeation chromatography (GPC), and scanning electron microscopy (SEM). The degree of substitution (DS) of the polysaccharide is 0.62, and the weight average molecular weight (Mw) is 1.34 × 104 Da. While retaining the morphological characteristics of polysaccharides, S-SGP appeared a large number of spherical structures and strong intermolecular forces. The in vitro activity study of S-SGP showed that the sulfated derivatives had the ability to scavenge DPPH radicals, hydroxyl radicals and superoxide anions, and the scavenging power tended to increase with the increase in polysaccharide concentration. It can inhibit the growth of human hepatoma cells (HepG2), human breast cancer cells (MDA-MB-231) and human non-small cell lung cancer cells (A549) in vitro. In addition, the treatment of A549 cells with sulfuric acid derivatives can decrease the mitochondrial membrane potential, induce apoptosis, and alter the expression of apoptosis-related mRNA and protein.
Funder
National Key Research and Development Program
General Plan of Shaanxi Province
Key Industrial Chain Projects of the Shaanxi Province Agricultural Field
Education Department of Shaanxi Province
Weiyang Technology Bureau
Xi’an City Innovation Plan Agricultural Field
Qinchuang Yuan “Scientists and Engineers” Team
Ningxia Zhong Ning Goji Industry Innovation Research Institute
Subject
Plant Science,Health Professions (miscellaneous),Health (social science),Microbiology,Food Science
Cited by
10 articles.
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