Abstract
Drops of cell suspension are seeded on nutrient medium containing 5% purified agar and spread between the agar surface and a cover glass. The liquid phase is absorbed by the concentrated agar, so that the cells are held in situ between glass and agar. After incubation nongerminated cells and micro-colonies are counted microscopically. Sporogenic capability is assessed by seeding very dense cell suspensions on acetate agar and covering with Teflon F.E.P. membrane which is permeable to oxygen.
Publisher
Canadian Science Publishing
Subject
Genetics,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Immunology,Microbiology
Cited by
5 articles.
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