Similarities between nonsporulated yeast cells and asci

Abstract

Yeast cells which did not sporulate in sporulation medium underwent some marked structural changes. Numerous vacuoles filled with material, possibly lipid, accumulated in the cytoplasm; the cell wall thickened and differentiated into an outer fibrillar and an inner particulate zone. The nonsporulated cells were viable but required 4–6 h at 27° to produce buds. In these respects the nonsporulated cells resembled asci or ascospores rather than vegetative cells.