Simulation and in vivo investigation of light-emitting diode, near infrared Gaussian beam profiles

Abstract

Near infrared spectroscopy is an optical imaging technique which offers a non-invasive, portable, and low-cost method for continuously measuring the oxygenation of tissues. In particular, it can provide the brain activation through measuring the blood oxygenation and blood volume in the cortex. Understanding and then improving the spatial and depth sensitivity of near infrared spectroscopy measurements to brain tissue are essential for designing experiments as well as interpreting research findings. In this study, we investigate the effect of applying two common light beam profiles including Uniform and Gaussian on the penetration depth of an LED-based near infrared spectroscopy. In this regard, two Gaussian profiles were produced by adjusting plano-convex and bi-convex lenses and the Uniform profile was provided by applying a flat lens. Two experiments were conducted in this study. First, a simulation experiment was carried out based on scanning the intra space of a liquid phantom by using static and pulsating absorbers to compare the penetration depth of the configurations applied on the LED-based near infrared spectroscopy with that of a laser-based near infrared spectroscopy. Second, to show the feasibility of the best proposed configuration applied, an in vivo experiment of stress assessment has been performed and its results have been compared with that results obtained by laser one. The results showed that the LED-based near infrared spectroscopy equipped with bi-convex lens provides a penetration depth and hence quality measurements of near infrared spectroscopy and its extracted heart rate variability signals as well as laser-based near infrared spectroscopy especially in the application of stress assessment.