Purification, Characterization, and Anticomplementary Activity of Polysaccharides From the Fruits of Amomum tsaoko Crevost & Lemarié

Author:

Hu Zhengyu1,Jin Long1,Zong Tieqiang1,Duan Yuanqi1,Zhang Xiaohui1,Yang Pengcheng1,Sun Jinfeng1,Zhou Wei1,Li Gao1ORCID

Affiliation:

1. Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Yanbian University, College of Pharmacy, Yanji, P.R. China

Abstract

The resin purification condition of polysaccharides from Amomum tsaoko Crevost et Lemarie (ATPs) was optimized using response surface method (RSM) and analytic hierarchy process (AHP): elution volume of 2 BV, flow rate of 2 BV/h, sample concentration of 2 mg/mL, sample volume of 0.4 BV, the comprehensive score of actual purification effect was 73.45 ± 0.32% (n = 3). Then, a homogenous polysaccharide (ATP-1-1) was isolated by DEAE-52 cellulose and Sephadex G-200 column. The structure of ATP-1-1 was characterized by the Fourier transform infrared spectroscopy, high-performance liquid chromatography, high-performance gel permeation chromatography, Congo red test, scanning electron microscopy, and thermogravimetric analysis, which showed that ATP-1-1 was composed of mannose, rhamnose, glucose, galactose, and arabinose in a molar ratio of 1.3:1.0:6.2:4.8:1.1 with molecular weight of 845.21 kDa, and had a triple-helix conformation, blocky microstructure, and good thermal stability. The evaluation of anti-complementary activity showed that ATP-1-1 possessed stronger anticomplementary activity than positive drug, significantly inhibited complement activation through both classical (CH50 = 6.28 ± 0.23 μg/mL) and alternative pathways (AP50 = 45.33 ± 0.16 μg/mL), and interacted with C2, C4, and factor B components in the activation cascade of the complement system.

Publisher

SAGE Publications

Subject

Complementary and alternative medicine,Plant Science,Drug Discovery,Pharmacology,General Medicine

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3