Precision RNAi using synthetic shRNAmir target sites-Reference-Cited by-同舟云学术

Precision RNAi using synthetic shRNAmir target sites

Published:2023-08-08 Issue: Volume:12 Page:
ISSN:2050-084X
Container-title:eLife
language:en
Short-container-title:

Author:

Hoffmann Thomas¹²^ORCID,Hörmann Alexandra³,Corcokovic Maja³,Zmajkovic Jakub¹^ORCID,Hinterndorfer Matthias¹^ORCID,Salkanovic Jasko³,Spreitzer Fiona³,Köferle Anna³^ORCID,Gitschtaler Katrin³,Popa Alexandra³,Oberndorfer Sarah³,Andersch Florian¹,Schaefer Markus¹,Fellner Michaela¹,Budano Nicole³,Ruppert Jan G³,Chetta Paolo³,Wurm Melanie³,Zuber Johannes¹⁴^ORCID,Neumüller Ralph A³^ORCID

Affiliation:

1. Research Institute of Molecular Pathology (IMP), Campus-Vienna-Biocenter 1

2. Vienna BioCenter PhD Program, Doctoral School of the University at Vienna and Medical University of Vienna, Vienna BioCenter (VBC)

3. Boehringer Ingelheim RCV GmbH & Co KG, Doktor-Boehringer-Gasse

4. Medical University of Vienna, Vienna BioCenter

Abstract

Loss-of-function genetic tools are widely applied for validating therapeutic targets, but their utility remains limited by incomplete on- and uncontrolled off-target effects. We describe artificial RNA interference (ARTi) based on synthetic, ultra-potent, off-target-free shRNAs that enable efficient and inducible suppression of any gene upon introduction of a synthetic target sequence into non-coding transcript regions. ARTi establishes a scalable loss-of-function tool with full control over on- and off-target effects.

Funder

Swiss National Science Foundation

Austrian Research Promotion Agency

Marie Skłodowska-Curie Actions

Publisher

eLife Sciences Publications, Ltd

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

Link

https://cdn.elifesciences.org/articles/84792/elife-84792-v1.pdf

Reference40 articles.

1. Paralogous synthetic lethality underlies genetic dependencies of the cancer-Mutated gene Stag2;Bailey;Life Science Alliance,2021