Determination of the minimum suppressing concentration to metronidazole in representatives of obligate and optional anaerobic microflora of periodontal pockets

Abstract

BACKGROUND: Due to the long period of use of metronidazole in medical dental practice, today it is relevant to study the sensitivity and the minimum inhibitory concentration of reference and clinical strains of obligate anaerobic microbiota to metronidazole in periodontitis. AIM: Determination of sensitivity and minimum suppressive concentration of reference and clinical strains of obligate anaerobic microbiota to metronidazole in periodontitis. MATERIALS AND METHODS: Strains of obligate anaerobic microbiota of periodontal pockets with moderate periodonitis were isolated from 30 patients. The study was carried out by the cassette micromethod, which is a modification of the method for determining the sensitivity by serial dilution in a dense agar medium. RESULTS: As a result of the study, it was found that many of the microbes found in periodontal pockets have a relatively low sensitivity to metronidazole (MIC from 4 to 12 g / ml). This circumstance is obviously due to the fact that this antibacterial chemotherapy drug has been used for a long time in periodontal practice. CONCLUSIONS: In this regard, in order to increase the effectiveness of local antibacterial therapy, this chemotherapy drug must be combined with antiseptics that have a pronounced antibacterial effect against periodontal pathogenic microflora, for example, Metrogyl Dent gel, containing, along with metronidazole, the antiseptic chlorhexidine, as well as the use of physical hardware methods of exposure to create significant the concentration of the antibacterial drug in the mucous membrane and in the contents of periodontal pockets, which can be done using phonophoresis.