Affiliation:
1. Gustavus Adolphus College
Abstract
Many high-performance liquid chromatography (HPLC) users are confronted with questions about how to improve upon the performance of an existing or recently developed method. These days, we have many technological options to consider, but how do we choose one (or a few) to try? The important variables that are most important for improving the speed of a separation are the maximum pressure available to drive the separation, and column temperature. Understanding the relationship between these variables and analysis time, and their effects on other choices made during method development, is helpful for developing methods that are both effective and time efficient.
Publisher
Multimedia Pharma Sciences, LLC
Reference9 articles.
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