What Are Options to Improve My Separation? Part III: Options to Consider to Improve Resolution of Complex Mixtures

Abstract

Many high performance liquid chromatography (HPLC) users are confronted with questions about how to improve upon the performance of an existing or recently developed method. These days, we have many technological options to consider, but how do we choose one (or a few) to try? The variables that are most important for improving the separation of complex samples are quite different from those that are most convenient for improving the resolution of simpler samples. Considering these differences can help avoid wasting time and resources when trying to improve separation performance.