What Are My Options to Improve My Separation? Part I: Foundational Concepts

Author:

Stoll Dwight R.1

Affiliation:

1. Gustavus Adolphus College

Abstract

Many high performance liquid chromatography (HPLC) users are confronted with questions about how to improve the performance of an existing or recently developed method. These days, we have many technological options to consider (for example, using higher pressures or temperatures, or both), but how do we choose one (or a few) to try? A systematic approach to method improvement can save time and resources by using methods that are likely to be better, and more robust, than those developed using a trial-and-error approach.

Publisher

Multimedia Pharma Sciences, LLC

Subject

Analytical Chemistry

Reference3 articles.

1. Purnell, J.H. The Correlation of Separating Power and Efficiency of Gas- chromatographic Columns. J. Chem. Soc. 1960, 1268–1274. https://doi.org/10.1039/jr9600001268.

2. L.R. Snyder; J.J. Kirkland; J.W. Dolan. Basic Concepts and the Control of Separation, in: Introduction to Modern Liquid Chromatography (Wiley, Hoboken, NJ, 3rd ed., 2010), pp. 69–71.

3. Sandra, P. and David, F. In Multidimensional Chromatography: Techniques and Applications, H. Cortes, Ed. (Marcel Dekker, New York, NY, 1990).

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