DNA Extraction for Sensitive Detection of Shiga Toxin-Producing Escherichia coli in Food by Real-Time PCR Assays
Author:
Affiliation:
1. Institute for Food and Environment Sciences Tokyo Kenbikyo-in Foundation
2. Division of Microbiology, National Institute of Health Sciences
Publisher
Japanese Society for Food Hygiene and Safety
Subject
General Medicine
Link
https://www.jstage.jst.go.jp/article/shokueishi/60/6/60_183/_pdf
Reference10 articles.
1. 1) Bosilevaca, J. M., Koohmaraie, M. Predicting the presence of Non-O157 Shiga toxin-producing Escherichia coli in ground beef by using molecular tests for Shiga toxins, intimin, and O serogroups. Appl. Environ. Microbiol, 78, 7152–7155 (2012)
2. 2) European Food Safety Authority. Scientific opinion of panel on biological hazards on monitoring of verotoxigenic Escherichia coli (VTEC) and identification of human pathogenic VTEC types. EFSA J., 579, 1–61 (2007)
3. 3) Fratamico, P. M., Bagi, L. K., Cray, W. C. Jr., Narang, N., Yan, X., Medina, M., Liu, Y. Detection by multiplex real-time polymerase chain reaction assays and isolation of Shiga toxin-producing Escherichia coli serogroups O26, O45, O103, O111, O121, and O145 in ground beef. Foodborne Pathog. Dis., 8, 601–607 (2011)
4. 4) European Food Safety Authority. Technical specifications for the monitoring and reporting of verotoxigenic Escherichia coli (VTEC) on animals and food (VTEC surveys on animals and food). EFSA J., 7, 1366 (2009)
5. 5) Hara-Kudo, Y., Kasuga, Y., Kiuchi, A., Horisaka, T., Kawasumi, T., Kumagi. S. Increased sensitivity in PCR detection of TDH-producing Vibrio parahaemolyticus in seafood using purified template DNA. J. Food Prot., 66, 1675–1680 (2003)
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