Predicting the Presence of Non-O157 Shiga Toxin-Producing Escherichia coli in Ground Beef by Using Molecular Tests for Shiga Toxins, Intimin, and O Serogroups

Author:

Bosilevac Joseph M.1,Koohmaraie Mohammad23

Affiliation:

1. USDA, ARS, U.S. Meat Animal Research Center, Clay Center, Nebraska, USA

2. IEH Laboratories and Consulting Group, Lake Forest Park, Washington, USA

3. College of Food and Agriculture, King Saud University, Riyadh, Saudi Arabia

Abstract

ABSTRACT When 3,972 ground beef enrichments with 6 confirmed to contain a non-O157 Shiga toxin-producing intimin-positive Escherichia coli isolate were tested for Shiga toxin, intimin, and O group (O26, O45, O103, O111, O121, and O145) genes, 183 potential positives and only 2 of the 6 confirmed positives were identified.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference20 articles.

1. Association of Escherichia coli O157:H7 tir polymorphisms with human infection;Bono JL;BMC Infect. Dis.,2007

2. BosilevacJMGueriniMNKoohmaraieM. 2009. Comparison of colony hybridization to phenotype screening on washed sheep's blood agar for the isolation of Shiga toxin producing Escherichia coli from complex matrixes, P02.5.3. Abstr.7th Int. Symp. Shiga Toxin (Verocytotoxin)-Producing Escherichia coli Infect.Buenos Aires, Argentina, 10 to 13 May 2009.

3. Prevalence and Characterization of Non-O157 Shiga Toxin-Producing Escherichia coli Isolates from Commercial Ground Beef in the United States

4. Non-O157 Shiga toxin-producing Escherichia coli infections in the United States, 1983-2002;Brooks JT;J. Infect. Dis.,2005

5. Molecular Analysis as an Aid To Assess the Public Health Risk of Non-O157 Shiga Toxin-Producing Escherichia coli Strains

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