Reconstruct a eukaryotic chromosome arm by de novo design and synthesis

Abstract

SUMMARYThe genome of an organism is inherited from its ancestor and keeps evolving over time, however, how much the current version could be altered remains unknown. Here, we use the left arm of chromosome XII (chrXIIL) as an example to probe the genome plasticity in Saccharomyces cerevisiae. A neochromosome was designed to harbor originally dispersed genes. The essentiality of sequences in chrXIIL was dissected by targeted DNA removal, chromosome truncation and random deletion. Notably, 12 genes were sufficient for survival, while 25 genes are required to retain robust fitness. Next, we demonstrated these genes could be reconstructed using synthetic regulatory sequences and recoded open-reading frames with “one-amino-acid-one-codon” strategy. Finally, we built a neochromsome, which could substitute for chrXIIL for cell viability, with these reconstructed genes. Our work not only highlights the high plasticity of yeast genome, but also illustrates the possibility of making functional chromosomes with completely artificial sequences.HIGHLIGHTSA neochromosome was designed to facilitate the assembly of exogenous DNA for stable expression in yeastThe left arm of chrXII could be minimized to just 12 genes to maintain viability, but additional genes were required to retain robust fitnessComprehensive recoding and transcriptional refactoring using artificial regulatory sequences produced a functional chromosome armA completely reconstructed neochromosome could replace the chrXIIL to maintain comparable fitness