Total Synthesis of a Functional Designer Eukaryotic Chromosome-Reference-Cited by-同舟云学术

Total Synthesis of a Functional Designer Eukaryotic Chromosome

Published:2014-04-04 Issue:6179 Volume:344 Page:55-58
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Annaluru Narayana¹,Muller Héloïse¹²³⁴,Mitchell Leslie A.²⁵,Ramalingam Sivaprakash¹,Stracquadanio Giovanni²⁶,Richardson Sarah M.⁶,Dymond Jessica S.²⁷,Kuang Zheng²,Scheifele Lisa Z.²⁸,Cooper Eric M.²,Cai Yizhi²⁹,Zeller Karen²,Agmon Neta²⁵,Han Jeffrey S.¹⁰,Hadjithomas Michalis¹¹,Tullman Jennifer⁶,Caravelli Katrina²¹²,Cirelli Kimberly¹¹²,Guo Zheyuan¹¹³,London Viktoriya¹¹³,Yeluru Apurva¹¹³,Murugan Sindurathy⁶,Kandavelou Karthikeyan¹¹⁴,Agier Nicolas¹⁵¹⁶,Fischer Gilles¹⁵¹⁶,Yang Kun²⁶,Martin J. Andrew²⁶,Bilgel Murat¹³,Bohutskyi Pavlo¹³,Boulier Kristin M.¹²,Capaldo Brian J.¹³,Chang Joy¹³,Charoen Kristie¹³,Choi Woo Jin¹³,Deng Peter¹¹,DiCarlo James E.¹³,Doong Judy¹³,Dunn Jessilyn¹³,Feinberg Jason I.¹²,Fernandez Christopher¹²,Floria Charlotte E.¹²,Gladowski David¹²,Hadidi Pasha¹³,Ishizuka Isabel¹²,Jabbari Javaneh¹²,Lau Calvin Y. L.¹³,Lee Pablo A.¹³,Li Sean¹³,Lin Denise¹²,Linder Matthias E.¹²,Ling Jonathan¹³,Liu Jaime¹³,Liu Jonathan¹³,London Mariya¹²,Ma Henry¹³,Mao Jessica¹³,McDade Jessica E.¹³,McMillan Alexandra¹²,Moore Aaron M.¹²,Oh Won Chan¹³,Ouyang Yu¹³,Patel Ruchi¹³,Paul Marina¹²,Paulsen Laura C.¹³,Qiu Judy¹³,Rhee Alex¹³,Rubashkin Matthew G.¹³,Soh Ina Y.¹²,Sotuyo Nathaniel E.¹²,Srinivas Venkatesh¹³,Suarez Allison¹³,Wong Andy¹³,Wong Remus¹³,Xie Wei Rose¹²,Xu Yijie¹³,Yu Allen T.¹²,Koszul Romain³⁴,Bader Joel S.²⁶,Boeke Jef D.²⁵¹¹,Chandrasegaran Srinivasan¹

Affiliation:

1. Department of Environmental Health Sciences, Johns Hopkins University (JHU) School of Public Health, Baltimore, MD 21205, USA.

2. High Throughput Biology Center, JHU School of Medicine, Baltimore, MD 21205, USA.

3. Group Spatial Regulation of Genomes, Department of Genomes Genetics, Institut Pasteur, F-75015 Paris, France.

4. CNRS, UMR 3525, F-75015 Paris, France.

5. New York University Langone Medical Center, New York, NY 10016, USA.

6. Department of Biomedical Engineering and Institute of Genetic Medicine, Whiting School of Engineering, JHU, Baltimore, MD 21218, USA.

7. Biological Sciences, Research and Exploratory Development Department, JHU Applied Physics Laboratory, Laurel, MD 20723, USA.

8. Department of Biology, Loyola University Maryland, Baltimore, MD 21210, USA.

9. University of Edinburgh, Edinburgh, Scotland, UK.

10. Carnegie Institution of Washington, Baltimore, MD 21218, USA.

11. Department of Biology, JHU, Baltimore, MD 21218, USA.

12. Krieger School of Arts and Sciences, JHU, Baltimore, MD 21218, USA.

13. Whiting School of Engineering, JHU, Baltimore, MD 21218, USA.

14. Pondicherry Biotech Private Limited, Pillaichavady, Puducherry 605014, India.

15. Sorbonne Universités, Université Pierre et Marie Curie, Univ Paris 06, UMR 7238, Génomique des Microorganismes, F-75005 Paris, France.

16. CNRS, UMR7238, Génomique des Microorganismes, F-75005 Paris, France.

Abstract

Designer Chromosome One of the ultimate aims of synthetic biology is to build designer organisms from the ground up. Rapid advances in DNA synthesis has allowed the assembly of complete bacterial genomes. Eukaryotic organisms, with their generally much larger and more complex genomes, present an additional challenge to synthetic biologists. Annaluru et al. (p. 55 , published online 27 March) designed a synthetic eukaryotic chromosome based on yeast chromosome III. The designer chromosome, shorn of destabilizing transfer RNA genes and transposons, is ∼14% smaller than its wild-type template and is fully functional with every gene tagged for easy removal.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference48 articles.

1. Life with 6000 Genes

2. Synthetic chromosome arms function in yeast and generate phenotypic diversity by design

3. The Saccharomyces cerevisiae SCRaMbLE system and genome minimization

4. Chemical Synthesis of Poliovirus cDNA: Generation of Infectious Virus in the Absence of Natural Template

5. Refactoring bacteriophage T7

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