Author:
,Temple Gary,Gerhard Daniela S.,Rasooly Rebekah,Feingold Elise A.,Good Peter J.,Robinson Cristen,Mandich Allison,Derge Jeffrey G.,Lewis Jeanne,Shoaf Debonny,Collins Francis S.,Jang Wonhee,Wagner Lukas,Shenmen Carolyn M.,Misquitta Leonie,Schaefer Carl F.,Buetow Kenneth H.,Bonner Tom I.,Yankie Linda,Ward Ming,Phan Lon,Astashyn Alex,Brown Garth,Farrell Catherine,Hart Jennifer,Landrum Melissa,Maidak Bonnie L.,Murphy Michael,Murphy Terence,Rajput Bhanu,Riddick Lillian,Webb David,Weber Janet,Wu Wendy,Pruitt Kim D.,Maglott Donna,Siepel Adam,Brejova Brona,Diekhans Mark,Harte Rachel,Baertsch Robert,Kent Jim,Haussler David,Brent Michael,Langton Laura,Comstock Charles L.G.,Stevens Michael,Wei Chaochun,van Baren Marijke J.,Salehi-Ashtiani Kourosh,Murray Ryan R.,Ghamsari Lila,Mello Elizabeth,Lin Chenwei,Pennacchio Christa,Schreiber Kirsten,Shapiro Nicole,Marsh Amber,Pardes Elizabeth,Moore Troy,Lebeau Anita,Muratet Mike,Simmons Blake,Kloske David,Sieja Stephanie,Hudson James,Sethupathy Praveen,Brownstein Michael,Bhat Narayan,Lazar Joseph,Jacob Howard,Gruber Chris E.,Smith Mark R.,McPherson John,Garcia Angela M.,Gunaratne Preethi H.,Wu Jiaqian,Muzny Donna,Gibbs Richard A.,Young Alice C.,Bouffard Gerard G.,Blakesley Robert W.,Mullikin Jim,Green Eric D.,Dickson Mark C.,Rodriguez Alex C.,Grimwood Jane,Schmutz Jeremy,Myers Richard M.,Hirst Martin,Zeng Thomas,Tse Kane,Moksa Michelle,Deng Merinda,Ma Kevin,Mah Diana,Pang Johnson,Taylor Greg,Chuah Eric,Deng Athena,Fichter Keith,Go Anne,Lee Stephanie,Wang Jing,Griffith Malachi,Morin Ryan,Moore Richard A.,Mayo Michael,Munro Sarah,Wagner Susan,Jones Steven J.M.,Holt Robert A.,Marra Marco A.,Lu Sun,Yang Shuwei,Hartigan James,Graf Marcus,Wagner Ralf,Letovksy Stanley,Pulido Jacqueline C.,Robison Keith,Esposito Dominic,Hartley James,Wall Vanessa E.,Hopkins Ralph F.,Ohara Osamu,Wiemann Stefan
Abstract
Since its start, the Mammalian Gene Collection (MGC) has sought to provide at least one full-protein-coding sequence cDNA clone for every human and mouse gene with a RefSeq transcript, and at least 6200 rat genes. The MGC cloning effort initially relied on random expressed sequence tag screening of cDNA libraries. Here, we summarize our recent progress using directed RT-PCR cloning and DNA synthesis. The MGC now contains clones with the entire protein-coding sequence for 92% of human and 89% of mouse genes with curated RefSeq (NM-accession) transcripts, and for 97% of human and 96% of mouse genes with curated RefSeq transcripts that have one or more PubMed publications, in addition to clones for more than 6300 rat genes. These high-quality MGC clones and their sequences are accessible without restriction to researchers worldwide.
Subject
Genetics(clinical),Genetics