Signature morpho-electric properties of diverse GABAergic interneurons in the human neocortex

Author:

Lee BrianORCID,Dalley Rachel,Miller Jeremy A,Chartrand Thomas,Close Jennie,Mann Rusty,Mukora Alice,Ng Lindsay,Alfiler Lauren,Baker Katherine,Bertagnolli DarrenORCID,Brouner KrissyORCID,Casper Tamara,Csajbok Eva,Dee Nick,Donadio Nicholas,Driessens Stan L.W.,Egdorf TomORCID,Enstrom Rachel,Galakhova Anna A,Gary AmandaORCID,Gelfand Emily,Goldy JeffORCID,Hadley Kristen,Heistek Tim S.,Hill Dijon,Johansen Nelson,Jorstad Nik,Kim Lisa,Kocsis Agnes Katalin,Kruse Lauren,Kunst Michael,Leon Gabriela,Long Brian,Mallory Matthew,Maxwell MichelleORCID,McGraw MedeaORCID,McMillen DelissaORCID,Melief Erica J,Molnar Gabor,Mortrud Marty T,Newman Dakota,Nyhus Julie,Opitz-Araya Ximena,Pham TrangthanhORCID,Pom AliceORCID,Potekhina Lydia,Rajanbabu Ram,Ruiz AugustinORCID,Sunkin Susan M,Szots Ildiko,Taskin Naz,Thyagarajan Bargavi,Tieu MichaelORCID,Trinh Jessica,Vargas Sara,Vumbaco David,Waleboer Femke,Weed Natalie,Williams Grace,Wilson Julia,Yao Shenqin,Zhou Thomas,Barzo Pal,Bakken Trygve,Cobbs Charles,Ellenbogen Richard G.,Esposito Luke,Ferreira Manuel,Gouwens Nathan W,Grannan Benjamin,Gwinn Ryder P.,Hauptman Jason S.,Hodge Rebecca,Jarsky Tim,Keene C.Dirk,Ko Andrew L.,Levi Boaz,Ojemann Jeffrey G.,Patel Anoop,Ruzevick Jacob,Silbergeld Daniel L.,Smith KimORCID,Waters Jack,Zeng Hongkui,Berg Jim,Goriounova Natalia A.,Kalmbach Brian,de Kock Christiaan P.J.,Mansvelder Huib D,Sorensen Staci A,Tamas Gabor,Lein Ed S.,Ting Jonathan T

Abstract

AbstractHuman cortical interneurons have been challenging to study due to high diversity and lack of mature brain tissue platforms and genetic targeting tools. We employed rapid GABAergic neuron viral labeling plus unbiased Patch-seq sampling in brain slices to define the signature morpho-electric properties of GABAergic neurons in the human neocortex. Viral targeting greatly facilitated sampling of the SST subclass, including primate specialized double bouquet cells which mapped to two SST transcriptomic types. Multimodal analysis uncovered an SST neuron type with properties inconsistent with original subclass assignment; we instead propose reclassification into PVALB subclass. Our findings provide novel insights about functional properties of human cortical GABAergic neuron subclasses and types and highlight the essential role of multimodal annotation for refinement of emerging transcriptomic cell type taxonomies.One Sentence SummaryViral genetic labeling of GABAergic neurons in humanex vivobrain slices paired with Patch-seq recording yields an in-depth functional annotation of human cortical interneuron subclasses and types and highlights the essential role of multimodal functional annotation for refinement of emerging transcriptomic cell type taxonomies.

Publisher

Cold Spring Harbor Laboratory

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3