A marmoset brain cell census reveals regional specialization of cellular identities-Reference-Cited by-同舟云学术

A marmoset brain cell census reveals regional specialization of cellular identities

Published:2023-10-13 Issue:41 Volume:9 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Krienen Fenna M.¹²^ORCID,Levandowski Kirsten M.²³⁴^ORCID,Zaniewski Heather²³⁴^ORCID,del Rosario Ricardo C.H.¹²,Schroeder Margaret E.²³⁴^ORCID,Goldman Melissa¹²,Wienisch Martin²³⁴^ORCID,Lutservitz Alyssa¹,Beja-Glasser Victoria F.²³⁴,Chen Cindy²³⁴^ORCID,Zhang Qiangge²³⁴^ORCID,Chan Ken Y.²,Li Katelyn X.³⁴^ORCID,Sharma Jitendra³⁴,McCormack Dana²³⁴^ORCID,Shin Tay Won³⁴⁵^ORCID,Harrahill Andrew³⁴^ORCID,Nyase Eric³⁴,Mudhar Gagandeep⁶,Mauermann Abigail³⁴⁵,Wysoker Alec²,Nemesh James²,Kashin Seva²,Vergara Josselyn²^ORCID,Chelini Gabriele⁷^ORCID,Dimidschstein Jordane²,Berretta Sabina⁸⁹^ORCID,Deverman Benjamin E.²^ORCID,Boyden Ed³⁴⁵^ORCID,McCarroll Steven A.¹²^ORCID,Feng Guoping²³

Affiliation:

1. Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.

2. Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.

3. McGovern Institute for Brain Research, Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.

4. Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

5. Howard Hughes Medical Institute, Cambridge, MA 02139, USA.

6. Princeton Neuroscience Institute, Princeton University, Princeton, NJ 08544, USA.

7. Center for Mind/Brain Sciences, University of Trento, Piazza della Manifattura n.1, Rovereto (TN) 38068, Italy.

8. Basic Neuroscience Division, McLean Hospital, Belmont, MA 02478, USA.

9. Department of Psychiatry, Harvard Medical School, Boston, MA 02115, USA.

Abstract

The mammalian brain is composed of many brain structures, each with its own ontogenetic and developmental history. We used single-nucleus RNA sequencing to sample over 2.4 million brain cells across 18 locations in the common marmoset, a New World monkey primed for genetic engineering, and examined gene expression patterns of cell types within and across brain structures. The adult transcriptomic identity of most neuronal types is shaped more by developmental origin than by neurotransmitter signaling repertoire. Quantitative mapping of GABAergic types with single-molecule FISH (smFISH) reveals that interneurons in the striatum and neocortex follow distinct spatial principles, and that lateral prefrontal and other higher-order cortical association areas are distinguished by high proportions of VIP + neurons. We use cell type–specific enhancers to drive AAV-GFP and reconstruct the morphologies of molecularly resolved interneuron types in neocortex and striatum. Our analyses highlight how lineage, local context, and functional class contribute to the transcriptional identity and biodistribution of primate brain cell types.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Link

https://www.science.org/doi/pdf/10.1126/sciadv.adk3986

Reference116 articles.

1. Z. Yao C. T. J. van Velthoven M. Kunst M. Zhang D. McMillen C. Lee W. Jung J. Goldy A. Abdelhak P. Baker E. Barkan D. Bertagnolli J. Campos D. Carey T. Casper A. B. Chakka R. Chakrabarty S. Chavan M. Chen M. Clark J. Close K. Crichton S. Daniel T. Dolbeare L. Ellingwood J. Gee A. Glandon J. Gloe J. Gould J. Gray N. Guilford J. Guzman D. Hirschstein W. Ho K. Jin M. Kroll K. Lathia A. Leon B. Long Z. Maltzer N. Martin R. McCue E. Meyerdierks T. N. Nguyen T. Pham C. Rimorin A. Ruiz N. Shapovalova C. Slaughterbeck J. Sulc M. Tieu A. Torkelson H. Tung N. V. Cuevas K. Wadhwani K. Ward B. Levi C. Farrell C. L. Thompson S. Mufti C. M. Pagan L. Kruse N. Dee S. M. Sunkin L. Esposito M. J. Hawrylycz J. Waters L. Ng K. A. Smith B. Tasic X. Zhuang H. Zeng A high-resolution transcriptomic and spatial atlas of cell types in the whole mouse brain. bioRxiv 2023.03.06.531121 [Preprint]. 2023 March 6. https://doi.org/10.1101/2023.03.06.531121.

2. K. Siletti R. Hodge A. M. Albiach L. Hu K. W. Lee P. Lönnerberg T. Bakken S.-L. Ding M. Clark T. Casper N. Dee J. Gloe C. Dirk Keene J. Nyhus H. Tung A. M. Yanny E. Arenas E. S. Lein S. Linnarsson Transcriptomic diversity of cell types across the adult human brain. Science 382 eadd7046 (2023).

3. Molecular, spatial, and functional single-cell profiling of the hypothalamic preoptic region

4. Molecular Diversity and Specializations among the Cells of the Adult Mouse Brain

5. Molecular Architecture of the Mouse Nervous System

Cited by 13 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Giant pyramidal neurons of the primary motor cortex express vasoactive intestinal polypeptide (VIP), a known marker of cortical interneurons;Scientific Reports;2024-09-11

2. Single-cell and spatial omics: exploring hypothalamic heterogeneity;Neural Regeneration Research;2024-07-10

3. A molecular and cellular perspective on human brain evolution and tempo;Nature;2024-06-19

4. Dopamine D1 receptor expression in dlPFC inhibitory parvalbumin neurons may contribute to higher visuospatial distractibility in marmosets versus macaques;2024-06-16

5. Transcriptomic landscape of mammalian ventral pallidum at single-cell resolution;2024-05-26