Author:
Nowotschin Sonja,Setty Manu,Kuo Ying-Yi,Lui Vincent,Garg Vidur,Sharma Roshan,Simon Claire S.,Saiz Nestor,Gardner Rui,Boutet Stéphane C.,Church Deanna M.,Hoodless Pamela A.,Hadjantonakis Anna-Katerina,Pe’er Dana
Abstract
AbstractTo comprehensively delineate the ontogeny of an organ system, we generated 112,217 singlecell transcriptomes representing all endoderm populations within the mouse embryo until midgestation. We employed graph-based approaches to model differentiating cells for spatio-temporal characterization of developmental trajectories. Our analysis reveals the detailed architecture of the emergence of the first (primitive or extra-embryonic) endodermal population and pluripotent epiblast. We uncover an unappreciated relationship between descendants of these lineages, before the onset of gastrulation, suggesting that mixing of extra-embryonic and embryonic endoderm cells occurs more than once during mammalian development. We map the trajectories of endoderm cells as they acquire embryonic versus extra-embryonic fates, and their spatial convergence within the gut endoderm; revealing them to be globally similar but retaining aspects of their lineage history. We observe the regionalized localization of cells along the forming gut tube, reflecting their extra-embryonic or embryonic origin, and their coordinate patterning into organ-specific territories along the anterior-posterior axis.
Publisher
Cold Spring Harbor Laboratory
Cited by
5 articles.
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