Author:
Michnick Stephen W.,Landry Christian R.,Levy Emmanuel D.,Diss Guillaume,Ear Po Hien,Kowarzyk Jacqueline,Malleshaiah Mohan K.,Messier Vincent,Tchekanda Emmanuelle
Abstract
Protein-fragment complementation assays (PCAs) comprise a family of assays that can be used to study protein–protein interactions (PPIs), conformation changes, and protein complex dimensions. We developed PCAs to provide simple and direct methods for the study of PPIs in any living cell, subcellular compartments or membranes, multicellular organisms, or in vitro. Because they are complete assays, requiring no cell-specific components other than reporter fragments, they can be applied in any context. PCAs provide a general strategy for the detection of proteins expressed at endogenous levels within appropriate subcellular compartments and with normal posttranslational modifications, in virtually any cell type or organism under any conditions. Here we introduce a number of applications of PCAs in budding yeast, Saccharomyces cerevisiae. These applications represent the full range of PPI characteristics that might be studied, from simple detection on a large scale to visualization of spatiotemporal dynamics.
Publisher
Cold Spring Harbor Laboratory
Subject
General Biochemistry, Genetics and Molecular Biology
Cited by
9 articles.
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