An optimized intein-mediated protein ligation approach for the efficient cyclization of cysteine-rich proteins
Author:
Publisher
Oxford University Press (OUP)
Subject
Molecular Biology,Biochemistry,Bioengineering,Biotechnology
Link
http://academic.oup.com/peds/article-pdf/27/12/481/17494903/gzu048.pdf
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1. Efficient splicing of the CPE intein derived from directed evolution of the <italic>Cryptococcus neoformans</italic> PRP8 intein;Acta Biochimica et Biophysica Sinica;2023-07-23
2. Heterologous expression of recombinant urate oxidase using the intein-mediated protein purification in Pichia pastoris;3 Biotech;2021-02-08
3. Intein-mediated backbone cyclization of entolimod confers enhanced radioprotective activity in mouse models;PeerJ;2018-06-18
4. Molecular Determinants for Protein Stabilization by Insertional Fusion to a Thermophilic Host Protein;ChemBioChem;2015-09-22
5. An efficient protocol towards site-specifically clickable nanobodies in high yield: cytoplasmic expression inEscherichia colicombined with intein-mediated protein ligation;Protein Engineering Design and Selection;2015-08-04
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