An efficient protocol towards site-specifically clickable nanobodies in high yield: cytoplasmic expression inEscherichia colicombined with intein-mediated protein ligation-Reference-Cited by-同舟云学术

An efficient protocol towards site-specifically clickable nanobodies in high yield: cytoplasmic expression inEscherichia colicombined with intein-mediated protein ligation

Published:2015-08-04 Issue:10 Volume:28 Page:351-363
ISSN:1741-0126
Container-title:Protein Engineering Design and Selection
language:en
Short-container-title:Protein Engineering, Design and Selection

Author:

Ta Duy Tien,Steen Redeker Erik,Billen Brecht,Reekmans Gunter,Sikulu Josephine,Noben Jean-Paul,Guedens Wanda,Adriaensens Peter

Funder

Fund for Scientific Research of Flanders

Publisher

Oxford University Press (OUP)

Subject

Molecular Biology,Biochemistry,Bioengineering,Biotechnology

Link

http://academic.oup.com/peds/article-pdf/28/10/351/17495265/gzv032.pdf

Reference75 articles.

1. Selection and identification of single domain antibody fragments from camel heavy-chain antibodies

2. Prokaryotic expression of antibodies

3. Introduction of unnatural amino acids into proteins using expressed protein ligation

4. Differential tumor-targeting abilities of three single-domain antibody formats

Cited by 37 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Streamlined on-column refolding and purification of nanobodies from inclusion bodies expressed as fusion proteins;Journal of Chromatography B;2024-10

2. Scalable, robust, high-throughput expression & purification of nanobodies enabled by 2-stage dynamic control;Metabolic Engineering;2024-09

3. Aptamers and Nanobodies as New Bioprobes for SARS-CoV-2 Diagnostic and Therapeutic System Applications;Biosensors;2024-03-15

4. Functionalized nanobody-based delivery systems for cancer diagnosis and therapeutic applications;Functionalized Nanomaterials for Cancer Research;2024

5. Scalable, Robust, High-throughput Expression, Purification & Characterization of Nanobodies Enabled by 2-Stage Dynamic Control;2023-12-14