Heterologous expression of recombinant urate oxidase using the intein-mediated protein purification in Pichia pastoris
Author:
Publisher
Springer Science and Business Media LLC
Subject
Agricultural and Biological Sciences (miscellaneous),Environmental Science (miscellaneous),Biotechnology
Link
http://link.springer.com/content/pdf/10.1007/s13205-021-02670-6.pdf
Reference63 articles.
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2. Ahmad M, Hirz M, Pichler H, Schwab H (2014) Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production. Appl Microbiol Biotechnol 98:5301–5317. https://doi.org/10.1007/s00253-014-5732-5
3. Alishah K, Asad S, Khajeh K, Akbari N (2016) Utilizing intein-mediated protein cleaving for purification of uricase, a multimeric enzyme. Enzyme Microb Technol 93:92–98. https://doi.org/10.1016/j.enzmictec.2016.08.001
4. Asad S, Dabirmanesh B, Khajeh K (2014) Phenol removal from refinery wastewater by mutant recombinant horseradish peroxidase. Biotechnol Appl Biochem 61:226–229. https://doi.org/10.1002/bab.1159
5. Azari M, Asad S, Mehrnia MR (2020) Heterologous production of porcine derived antimicrobial peptide PR-39 in Escherichia coli using SUMO and intein fusion systems. Protein Expr Purif 169:105568. https://doi.org/10.1016/j.pep.2020.105568
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