High yield expression and purification of Aspergillus flavus uricase cloned in Pichia pink™ expression system
Author:
Publisher
Springer Science and Business Media LLC
Subject
Agricultural and Biological Sciences (miscellaneous),Environmental Science (miscellaneous),Biotechnology
Link
https://link.springer.com/content/pdf/10.1007/s13205-023-03710-z.pdf
Reference24 articles.
1. Alishah K, Asad S, Khajeh K, Akbari N (2016) Utilizing intein-mediated protein cleaving for purification of uricase, a multimeric enzyme. Enzyme Microb Technol 93–94:92–98. https://doi.org/10.1016/j.enzmictec.2016.08.001
2. Askri H, Akrouti I, Rourou S, Kallèl H (2022) Production, purification, and characterization of recombinant rabies virus glycoprotein expressed in PichiaPink™ yeast. Biotechnol Rep (Amst). 35:e00736. https://doi.org/10.1016/j.btre.2022.e00736. PMID: 35646619; PMCID: PMC9130087.
3. Bayol A, Capdevielle J, Malazzi P, Buzy A, Claude Bonnet M, Colloc’h N, Mornon JP, Loyaux D, Ferrara P (2002) Modification of a reactive cysteine explains differences between rasburicase and Uricozyme, a natural Aspergillus flavus uricase. Biotechnol Appl Biochem 36(1):21–31. https://doi.org/10.1042/ba20010083. (PMID: 12149119)
4. Chen Z, Wang Z, He X, Guo X, Li W, Zhang B (2008) Uricase production by a recombinant Hansenula polymorpha strain harboring Candida utilis uricase gene. Appl Microbiol Biotechnol 79(4):545–554. https://doi.org/10.1007/s00253-008-1472-8
5. Chevalet L, Tiraby G, Cabane B, Loison G (1992) Transformation of Aspergillus flavus: construction of urate oxidase-deficient mutants by gene disruption. Curr Genet 21(6):447–453. https://doi.org/10.1007/BF00351654. (PMID: 1617733)
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