Long-read and chromosome-scale assembly of the hexaploid wheat genome achieves high resolution for research and breeding

Author:

Aury Jean-Marc1ORCID,Engelen Stefan1ORCID,Istace Benjamin1ORCID,Monat Cécile2ORCID,Lasserre-Zuber Pauline2,Belser Caroline1ORCID,Cruaud Corinne3ORCID,Rimbert Hélène2ORCID,Leroy Philippe2ORCID,Arribat Sandrine4ORCID,Dufau Isabelle4,Bellec Arnaud4ORCID,Grimbichler David5ORCID,Papon Nathan2,Paux Etienne2ORCID,Ranoux Marion2,Alberti Adriana1ORCID,Wincker Patrick1ORCID,Choulet Frédéric2ORCID

Affiliation:

1. Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Univ Evry, Université Paris-Saclay, 91057 Evry, France

2. GDEC, Université Clermont Auvergne, INRAE, UMR1095, 63000 Clermont-Ferrand, France

3. Commissariat à l'Energie Atomique (CEA), Institut François Jacob, Genoscope, F-91057 Evry, France

4. INRAE, CNRGV French Plant Genomic Resource Center, F-31320, Castanet Tolosan, France

5. Mésocentre Clermont Auvergne, DOSI / Bâtiment Turing, 7 avenue Blaise Pascal, 63178 Aubière, France

Abstract

Abstract Background The sequencing of the wheat (Triticum aestivum) genome has been a methodological challenge for many years owing to its large size (15.5 Gb), repeat content, and hexaploidy. Many initiatives aiming at obtaining a reference genome of cultivar Chinese Spring have been launched in the past years and it was achieved in 2018 as the result of a huge effort to combine short-read sequencing with many other resources. Reference-quality genome assemblies were then produced for other accessions, but the rapid evolution of sequencing technologies offers opportunities to reach high-quality standards at lower cost. Results Here, we report on an optimized procedure based on long reads produced on the Oxford Nanopore Technology PromethION device to assemble the genome of the French bread wheat cultivar Renan. Conclusions We provide the most contiguous chromosome-scale assembly of a bread wheat genome to date. Coupled with an annotation based on RNA-sequencing data, this resource will be valuable for the crop community and will facilitate the rapid selection of agronomically important traits. We also provide a framework to generate high-quality assemblies of complex genomes using ONT.

Funder

China Earthquake Administration

Publisher

Oxford University Press (OUP)

Subject

Computer Science Applications,Health Informatics

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3