Mapping developmental paths of monkey primordial germ-like cells differentiation from pluripotent stem cells by single cell ribonucleic acid sequencing analysis

Author:

Zhang Puyao12,Xue Sengren3,Guo Rongrong45,Liu Jian45,Bai Bing45,Li Dexuan45,Hyraht Ahjol45,Sun Nianqin45,Shao Honglian45,Fan Yong67,Ji Weizhi45,Yang Shihua3,Yu Yang12,Tan Tao45

Affiliation:

1. Center for Reproductive Medicine , Department of Obstetrics and Gynecology, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology and Key Laboratory of Assisted Reproduction, Ministry of Education, , Beijing , China

2. Peking University Third Hospital , Department of Obstetrics and Gynecology, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology and Key Laboratory of Assisted Reproduction, Ministry of Education, , Beijing , China

3. College of Veterinary Medicine, South China Agricultural University , Guangzhou , China

4. State Key Laboratory of Primate Biomedical Research , Institute of Primate Translational Medicine, , Kunming , China

5. Kunming University of Science and Technology , Institute of Primate Translational Medicine, , Kunming , China

6. Department of Gynecology and Obstetrics , Key Laboratory for Major Obstetric Diseases of Guangdong Province, , Guangzhou , China

7. The Third Affiliated Hospital of Guangzhou Medical University , Key Laboratory for Major Obstetric Diseases of Guangdong Province, , Guangzhou , China

Abstract

Abstract The induction of primordial germ-like cells (PGCLCs) from pluripotent stem cells (PSCs) provides a powerful system to study the cellular and molecular mechanisms underlying germline specification, which are difficult to study in vivo. The studies reveal the existence of a species-specific mechanism underlying PGCLCs between humans and mice, highlighting the necessity to study regulatory networks in more species, especially in primates. Harnessing the power of single-cell RNA sequencing (scRNA-seq) analysis, the detailed trajectory of human PGCLCs specification in vitro has been achieved. However, the study of nonhuman primates is still needed. Here, we applied an embryoid body (EB) differentiation system to induce PGCLCs specification from cynomolgus monkey male and female PSCs, and then performed high throughput scRNA-seq analysis of approximately 40 000 PSCs and cells within EBs. We found that EBs provided a niche for PGCLCs differentiation by secreting growth factors critical for PGCLC specification, such as bone morphogenetic protein 2 (BMP2), BMP4, and Wnt Family Member 3. Moreover, the developmental trajectory of PGCLCs was reconstituted, and gene expression dynamics were revealed. Our study outlines the roadmap of PGCLC specification from PSCs and provides insights that will improve the differentiation efficiency of PGCLCs from PSCs.

Funder

National Key Research and Development Program of China

National Natural Science Foundation of China

Yunnan Fundamental Research Projects

Publisher

Oxford University Press (OUP)

Subject

Cell Biology,General Medicine,Reproductive Medicine

Reference42 articles.

1. Epigenetic events in mammalian germ-cell development: reprogramming and beyond;Sasaki;Nat Rev Genet,2008

2. Primordial germ-cell development and epigenetic reprogramming in mammals;Leitch;Curr Top Dev Biol,2013

3. Primordial germ cell specification: a context-dependent cellular differentiation event [corrected];Gunesdogan;Philos Trans R Soc Lond B Biol Sci,2014

4. How to make a primordial germ cell;Magnusdottir;Development,2014

5. Specification and epigenetic programming of the human germ line;Tang;Nat Rev Genet,2016

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