Rapid Enzyme-Linked Immunoassay for Detection of Salmonella in Food and Feed Products: Performance Testing Program

Author:

Bolton Frederick J1,Fritz Erica1,Poynton Sharon1,Jensen Torben2

Affiliation:

1. Royal Preston Hospital, Public Health Laboratory, PO Box 202, Sharon Green Ln, Preston, PR2 9HG, United Kingdom

2. Bioline, Polarvej 60, DK-7100, Denmark

Abstract

Abstract The BIOLINE Salmonella ELISA Test for Salmonella spp., which is a rapid, easy, and convenient assay was evaluated for use in detecting Salmonella in foods and feeds. Each food matrix or feed was artificially contaminated with low levels of Salmonella. Twenty different matrixes were studied and 20 different Salmonella strains from a broad variety of serogroups (B, C, D, E, F, G, H, I, M, O, P, and U) were used. The ELISA Test kit detected levels as low as 1 cfu/25 g sample with at least 4 of the 20 matrixes tested. The test kit is applicable to all sample types tested. The BIOLINE Salmonella ELISA Test kit has been granted AOAC–RI performance tested status.

Publisher

Oxford University Press (OUP)

Subject

Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry

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