An improved method for the determination of dissolved nitric oxide (NO) in seawater samples
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Published:2015-11-27
Issue:6
Volume:11
Page:937-946
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ISSN:1812-0792
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Container-title:Ocean Science
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language:en
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Short-container-title:Ocean Sci.
Author:
Lutterbeck H. E., Bange H. W.ORCID
Abstract
Abstract. Nitric oxide (NO) is a short-lived intermediate of the oceanic nitrogen cycle; however, due to its high reactivity, measurements of dissolved NO in seawater are rare. Here we present an improved method to determine NO concentrations in discrete seawater samples. The set-up of our system consisted of a chemiluminescence NO analyser connected to a stripping unit. The limit of detection for our method is 5 pmol NO in aqueous solution, which translates into 0.25 nmol L−1 when using a 20 mL seawater sample volume. Our method was applied to measure high-resolution depth profiles of dissolved NO during a cruise to the eastern tropical South Pacific Ocean. It is fast and comparably easy to handle; thus it opens the door for investigating the distribution of NO in the ocean, and it facilitates laboratory studies on NO pathways.
Publisher
Copernicus GmbH
Subject
Cell Biology,Developmental Biology,Embryology,Anatomy
Reference48 articles.
1. Adeleke, B. B. and Wan, J. K. S.: Further ESR evidence of the primary N-N cleavage in the photolysis of dimethylnitrosamine: Indirect spin trapping of the primary radical NO, Mol. Photochem., 6, 329–331, 1974. 2. Alarcón, G. and Ulloa, O.: Cruise Report, MOOMZ-2, Departamento de Oceanografía, Universidad de Concepción, Concepción, Chile, 28 pp., 2009. 3. Alpert, C., Ramdev, N., George, D., and Loscalzo, J.: Detection of S-nitrosothiols and other nitric oxide derivatives by photolysis-chemiluminescence spectrometry, Anal. Biochem., 245, 1–7, 1997. 4. Bange, H. W.: Gaseous nitrogen compounds (NO, N2O, N2, NH3) in the ocean, in: Nitrogen in the Marine Environment, 2, edited by: Capone, D. G., Bronk, D. A., Mulholland, M. R., and Carpenter, E. J., Elsevier, Amsterdam, the Netherlands, 51–94, 2008 5. Canfield, D. E., Stewart, F. J., Thamdrup, B., De Brabandere, L., Dalsgaard, T., Delong, E. F., Revsbech, N. P., and Ulloa, O.: A cryptic sulfur cycle in oxygen-minimum–zone waters off the Chilean coast, Science, 330, 1375–1378, 2010.
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