Development of a Colloidal Gold-Based Immunochromatographic Assay for the Rapid Detection of Aflatoxin B1 in Food Samples

Author:

Chen Xue Qing1,Lu Sha Sha1,Sun Qing1,Yang Jing Chen1,Shi Guo Qing1

Affiliation:

1. University of Science and Technology Beijing

Abstract

A rapid and sensitive competitive immunochromatographic assay for aflatoxin B1 (AFB1) based on colloidal gold-antibody probe was developed. The limit of detection for AFB1 standard solution was 0.5ng/ml and for foodstuff sample was 10μg/Kg. Other aflatoxins including AFM1, AFB2, AFG1, and AFG2 have less cross-reactivity to the strip. The storge life of the strip was at least 12 monthes. The method was well agreement with the ELISA method by testing food samples. This method is suitable for rapid testing of AFB1 on site.

Publisher

Trans Tech Publications, Ltd.

Subject

General Engineering

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