Establishment of a Cleavage-Based Single-Plasmid Dual-Luciferase Surrogate Reporter for the Cleavage Efficiency Evaluation of CRISPR-Cas12a Systems and Its Primary Application-Reference-Cited by-同舟云学术

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Establishment of a Cleavage-Based Single-Plasmid Dual-Luciferase Surrogate Reporter for the Cleavage Efficiency Evaluation of CRISPR-Cas12a Systems and Its Primary Application

Published:2024-06-01 Issue:3 Volume:7 Page:156-167
ISSN:2573-1599
Container-title:The CRISPR Journal
language:en
Short-container-title:The CRISPR Journal

Author:

Shi Yaoqiang¹^ORCID,Tan Qi¹,Yang Chunhui¹,Li Shilin¹,Li Yujia¹,He Baoren²,Xie He³,Duan Xiaoqiong¹,Chen Limin¹²³

Affiliation:

1. Provincial Key Laboratory for Transfusion-Transmitted Infectious Diseases, Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, China.

2. The Joint Laboratory on Transfusion-Transmitted Diseases (TTDs) Between Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Nanning Blood Center, Nanning Blood Center, Nanning, China.

3. The Hospital of Xidian Group, Xi’an, China.

Publisher

Mary Ann Liebert Inc

Link

https://www.liebertpub.com/doi/pdf/10.1089/crispr.2024.0038

Reference32 articles.

1. CRISPR/dCas9‐mediated activation of multiple endogenous target genes directly converts human foreskin fibroblasts into Leydig‐like cells

2. Loosely-packed dynamical structures with partially-melted surface being the key for thermophilic argonaute proteins achieving high DNA-cleavage activity

3. CRISPR technology: A decade of genome editing is only the beginning

4. Global chromosome rearrangement induced by CRISPR-Cas9 reshapes the genome and transcriptome of human cells

5. A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

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