Tamarind Multifunctional Protein: Safety and Anti-Inflammatory Potential in Intestinal Mucosa and Adipose Tissue in a Preclinical Model of Diet-Induced Obesity

Author:

Lima Vanessa C.O.,Luz Anna B.S.,Amarante Maria do Socorro M.,Lima Maíra C.J.S.,Carvalho Fabiana M.C.,Figueredo Julia B.S.,Santos Pedro P.A.,Camillo Christina S.,Ladd Fernando V.L.,Maciel Bruna L.L.,Uchôa Adriana F.,Morais Ana H.A.

Abstract

<b><i>Introduction:</i></b> Obesity has emerged as one of the main public health problems. This condition triggers a series of hormonal and metabolic changes related to a low-grade chronic inflammatory condition. The trypsin inhibitor purified from tamarind (TTIp) seeds is a promising anti-inflammatory molecule, but its safety needs to be evaluated. This study aimed to evaluate TTIp bioactive dose effects on organs involved in its metabolism (liver and pancreas) and affected tissues (small intestine and perirenal adipose tissue) in an obesity model. <b><i>Methods:</i></b> Three groups of adult male Wistar rats were used (n = 5). Two of these groups had diet-induced obesity, and a third group was eutrophic. TTIp was administered by gavage in one of the obese groups for 10 days, while the remaining groups received a vehicle. The chromatographic profile and the inhibition assay corroded the purification of the inhibitor. Physical and behavioral changes, liver enzymes, and stereological and histopathological analyses of tissues were evaluated. <b><i>Results:</i></b> TTIp did not cause visible signs of toxicity, nor caused changes in liver enzymes, the liver, and pancreatic tissues. TTIp did not cause changes in the intestinal mucosa, showing improvement in the villi’s histopathological characteristics compared to the group of animals with obesity without treatment with TTIp (<i>p</i> = 0.004). The analysis of perirenal adipose tissue showed that the average sectional area of animals with obesity that received TTIp did not differ from the control. There was a difference between the high glycemic load diet group and the group treated with the inhibitor (351.8 ± 55.5) (<i>p</i> = 0.016). In addition, the group that received TTIp had no inflammatory infiltrates. <b><i>Conclusion:</i></b> Based on histological and stereological analysis, the use of TTIp is potentially safe and anti-inflammatory in the evaluated obesity model and can be investigated as a possible adjuvant in obesity therapy.

Publisher

S. Karger AG

Subject

Physiology (medical),Health(social science)

Reference24 articles.

1. Srivastava G, Apovian C. Future pharmacotherapy for obesity: new anti-obesity drugs on the horizon. Curr Obes Rep. 2018 Mar;7(2):147–61.

2. Qi RF, Song ZW, Chi CW. Structural features and molecular evolution of bowman-birk protease inhibitors and their potential application. Acta Biochim Biophys Sin. 2005 May;37(5):283–92.

3. Ribeiro JA, Serquiz AC, Silva PF, Barbosa PB, Sampaio TB, Araújo Junior RF, et al. Trypsin inhibitor from Tamarindus indica L. seeds reduces weight gain and food consumption and increases plasmatic cholecystokinin levels. Clinics. 2015 Feb;70(2):136–43.

4. Carvalho FM, Lima VC, Costa IS, Medeiros AF, Serquiz AC, Lima MC, et al. A trypsin inhibitor from tamarind reduces food intake and improves inflammatory status in rats with metabolic syndrome regardless of weight loss. Nutrients. 2016 Oct;8(10):544.

5. Costa IS, Medeiros AF, Carvalho FMC, Lima VCO, Serquiz RP, Serquiz AC, et al. Satietogenic protein from tamarind seeds decreases food intake, leptin plasma and CCK-1r gene expression in obese wistar rats. Obes Facts. 2018 Dec;11(6):440–53.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3